Citizen scientists sampled more than 600 rivers, streams, lakes and ponds across Germany. The samples have now been analysed by researchers under the auspices of IGB. In this way, a unique data set emerged that provides evidence of three Germany-wide trends: first, microorganisms in the sediment show signs of chemical stress and antibiotic resistance in their genetic material. Second, excessive artificial lighting at night alters the species composition of underwater microorganisms. And third, all studied water bodies emit greenhouse gases.